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1 Interdisciplinary Program in Bioinformatics, Seoul National University, 56-1 Shillim-dong, Kwanak-gu, Seoul 151-742, Republic of Korea
2 School of Biological Sciences and Institute of Microbiology, Seoul National University, 56-1 Shillim-dong, Kwanak-gu, Seoul 151-742, Republic of Korea
Correspondence
Jongsik Chun
jchun{at}snu.ac.kr
16S rRNA gene sequences have been widely used for the identification of prokaryotes. However, the flood of sequences of non-type strains and the lack of a peer-reviewed database for 16S rRNA gene sequences of type strains have made routine identification of isolates difficult and labour-intensive. In the present study, we generated a database containing 16S rRNA gene sequences of all prokaryotic type strains. In addition, a web-based tool, named EzTaxon, for analysis of 16S rRNA gene sequences was constructed to achieve identification of isolates based on pairwise nucleotide similarity values and phylogenetic inference methods. The system developed provides users with a similarity-based search, multiple sequence alignment and various phylogenetic analyses. All of these functions together with the 16S rRNA gene sequence database of type strains can be successfully used for automated and reliable identification of prokaryotic isolates. The EzTaxon server is freely accessible over the Internet at http://www.eztaxon.org/
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L. Ponnusamy, N. Xu, S. Nojima, D. M. Wesson, C. Schal, and C. S. Apperson Identification of bacteria and bacteria-associated chemical cues that mediate oviposition site preferences by Aedes aegypti PNAS, July 8, 2008; 105(27): 9262 - 9267. [Abstract] [Full Text] [PDF] |
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M. Cai, X.-Y. Zhi, S.-K. Tang, Y.-Q. Zhang, L.-H. Xu, and W.-J. Li Streptomonospora halophila sp. nov., a halophilic actinomycete isolated from a hypersaline soil Int J Syst Evol Microbiol, July 1, 2008; 58(7): 1556 - 1560. [Abstract] [Full Text] [PDF] |
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S. W. Roh, Z.-X. Quan, Y.-D. Nam, H.-W. Chang, K.-H. Kim, M.-K. Kim, W.-T. Im, L. Jin, S.-H. Kim, S.-T. Lee, et al. Pedobacter agri sp. nov., from soil Int J Syst Evol Microbiol, July 1, 2008; 58(7): 1640 - 1643. [Abstract] [Full Text] [PDF] |
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S. Jit, M. Dadhwal, O. Prakash, and R. Lal Flavobacterium lindanitolerans sp. nov., isolated from hexachlorocyclohexane-contaminated soil Int J Syst Evol Microbiol, July 1, 2008; 58(7): 1665 - 1669. [Abstract] [Full Text] [PDF] |
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K. H. Cho, S. G. Hong, H. H. Cho, Y. K. Lee, J. Chun, and H. K. Lee Maribacter arcticus sp. nov., isolated from Arctic marine sediment Int J Syst Evol Microbiol, June 1, 2008; 58(6): 1300 - 1303. [Abstract] [Full Text] [PDF] |
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S. G. Dastager, J.-C. Lee, Y.-J. Ju, D.-J. Park, and C.-J. Kim Marmoricola bigeumensis sp. nov., a member of the family Nocardioidaceae Int J Syst Evol Microbiol, May 1, 2008; 58(5): 1060 - 1063. [Abstract] [Full Text] [PDF] |
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L.-J. Yuan, Y.-Q. Zhang, Y. Guan, Y.-Z. Wei, Q.-P. Li, L.-Y. Yu, W.-J. Li, and Y.-Q. Zhang Saccharopolyspora antimicrobica sp. nov., an actinomycete from soil Int J Syst Evol Microbiol, May 1, 2008; 58(5): 1180 - 1185. [Abstract] [Full Text] [PDF] |
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J.-T. Wang, Y.-J. Chou, J.-H. Chou, C. A. Chen, and W.-M. Chen Tenacibaculum aiptasiae sp. nov., isolated from a sea anemone Aiptasia pulchella Int J Syst Evol Microbiol, April 1, 2008; 58(4): 761 - 766. [Abstract] [Full Text] [PDF] |
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M. Pineiro-Vidal, C. G. Carballas, O. Gomez-Barreiro, A. Riaza, and Y. Santos Tenacibaculum soleae sp. nov., isolated from diseased sole (Solea senegalensis Kaup) Int J Syst Evol Microbiol, April 1, 2008; 58(4): 881 - 885. [Abstract] [Full Text] [PDF] |
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Y.-J. Chou, J.-H. Chou, K.-Y. Lin, M.-C. Lin, Y.-H. Wei, A. B. Arun, C.-C. Young, and W.-M. Chen Rothia terrae sp. nov. isolated from soil in Taiwan Int J Syst Evol Microbiol, January 1, 2008; 58(1): 84 - 88. [Abstract] [Full Text] [PDF] |
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J.-H. Kim, K.-Y. Kim, Y.-T. Hahm, B.-S. Kim, J. Chun, and C.-J. Cha Actibacter sediminis gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from tidal flat sediment Int J Syst Evol Microbiol, January 1, 2008; 58(1): 139 - 143. [Abstract] [Full Text] [PDF] |
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M. Okamoto, Y. Benno, K.-P Leung, and N. Maeda Bifidobacterium tsurumiense sp. nov., from hamster dental plaque Int J Syst Evol Microbiol, January 1, 2008; 58(1): 144 - 148. [Abstract] [Full Text] [PDF] |
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M. E. Trujillo, R. M. Kroppenstedt, C. Fernandez-Molinero, P. Schumann, and E. Martinez-Molina Micromonospora lupini sp. nov. and Micromonospora saelicesensis sp. nov., isolated from root nodules of Lupinus angustifolius Int J Syst Evol Microbiol, December 1, 2007; 57(12): 2799 - 2804. [Abstract] [Full Text] [PDF] |
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S.-C. Chang, W.-M. Chen, J.-T. Wang, and M.-C. Wu Chitinilyticum aquatile gen. nov., sp. nov., a chitinolytic bacterium isolated from a freshwater pond used for Pacific white shrimp culture Int J Syst Evol Microbiol, December 1, 2007; 57(12): 2854 - 2860. [Abstract] [Full Text] [PDF] |
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J.-H. Chou, K.-Y. Lin, M.-C. Lin, S.-Y. Sheu, Y.-H. Wei, A. B. Arun, C.-C. Young, and W.-M. Chen Brachybacterium phenoliresistens sp. nov., isolated from oil-contaminated coastal sand Int J Syst Evol Microbiol, November 1, 2007; 57(11): 2674 - 2679. [Abstract] [Full Text] [PDF] |
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