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International Journal of Systematic and Evolutionary Microbiology, Vol 51, 1315-1322, Copyright © 2001 by Society for General Microbiology


DNA--DNA hybridization study of Bradyrhizobium strains

A Willems, F Doignon-Bourcier, J Goris, R Coopman, P de Lajudie, P De Vos and M Gillis
Laboratorium voor Microbiologie, Faculteit Wetenschappen, Universiteit Gent, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium

DNA--DNA hybridizations were performed between Bradyrhizobium strains, isolated mainly from Faidherbia albida and Aeschynomene species, as well as Bradyrhizobium reference strains. Results indicated that the genus Bradyrhizobium consists of at least 11 genospecies, I to XI. The genospecies formed four subgeneric groups that were more closely related to each other (> 40% DNA hybridization) than to other genospecies (< 40% DNA hybridization): (i) genospecies I (Bradyrhizobium japonicum), III (Bradyrhizobium liaoningense), IV and V; (ii) genospecies VI and VIII; (iii) genospecies VII and IX; and (iv) genospecies II (Bradyrhizobium elkanii), X and XI. Photosynthetic Aeschynomene isolates were found to belong to at least two distinct genospecies in one subgeneric group. DNA--DNA hybridization data are compared with data from amplified fragment length polymorphism analysis and 16S--23S rDNA spacer sequence analysis.


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C. Baginsky, B. Brito, J. Imperial, J.-M. Palacios, and T. Ruiz-Argueso
Diversity and Evolution of Hydrogenase Systems in Rhizobia
Appl. Envir. Microbiol., October 1, 2002; 68(10): 4915 - 4924.
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