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1Laboratory of Microbiology, Universiteit Gent, Ghent, United Kingdom
2Department of Medical Microbiology, University Hospital UIA, Antwerp, United Kingdom
4Laboratory of Cellular Immunology, Vlaams Instituut voor Biotechnologie, Vrije Universiteit Brussel, Sint-Genesius-Rode, United Kingdom
5Department of Microbiology, Academisch Ziekenhuis, Vrije Universiteit Brussel, Brussels, Belgium, United Kingdom
3National Collection of Type Cultures, Central Public Health Laboratory, London NW9 5HT, United Kingdom
6 Department of Medical Microbiology, University of Edinburgh Medical School, Edinburgh, United Kingdom
* Corresponding author. Mailing address:Laboratorium voor Microbiologie, Ledeganckstraat 35, B-9000 Ghent, Belgium. Phone: (32)9.264.51.13. Fax: (32)9.264.50.92. E-mail: Peter.Vandamme{at}rug.ac.be.
ABSTRACT
We performed an integrated genotypic and phenotypic analysis of 128 strains of the genera Burkholderia, Ralstonia, and Pseudomonas in order to study the taxonomic structure of Burkholderia cepacia and its relationships with other Burkholderia species. Our data show that presumed B. cepacia strains isolated from cystic fibrosis patients belong to at least five distinct genomic species, one of which was identified as Burkholderia vietnamiensis. This group of five phenotypically similar species is referred to as the B. cepacia complex. The name Burkholderia multivorans is proposed for one of these genomic species, which was formerly referred to as B. cepacia genomovar II; the remaining B. cepacia groups are referred to as genomovars I, III, and IV, pending additional differential phenotypic tests. The role and pathogenic potential of each of these taxa, particularly in view of the potentially fatal infections in cystic fibrosis patients, need further evaluation. The data presented also demonstrate that Pseudomonas glathei and Pseudomonas pyrrocinia should be reclassified as Burkholderia species.
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T. Coenye, J. Goris, P. De Vos, P. Vandamme, and J. J. LiPuma Classification of Ralstonia pickettii-like isolates from the environment and clinical samples as Ralstonia insidiosa sp. nov. Int J Syst Evol Microbiol, July 1, 2003; 53(4): 1075 - 1080. [Abstract] [Full Text] [PDF]
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L. Liu, T. Spilker, T. Coenye, and J. J. LiPuma Identification by Subtractive Hybridization of a Novel Insertion Element Specific for Two Widespread Burkholderia cepacia Genomovar III Strains J. Clin. Microbiol., June 1, 2003; 41(6): 2471 - 2476. [Abstract] [Full Text] [PDF]
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H. Komatsu, Y. Imura, A. Ohori, Y. Nagata, and M. Tsuda Distribution and Organization of Auxotrophic Genes on the Multichromosomal Genome of Burkholderia multivorans ATCC 17616 J. Bacteriol., June 1, 2003; 185(11): 3333 - 3343. [Abstract] [Full Text] [PDF]
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R. Langley, D. T. Kenna, P. Vandamme, R. Ure, and J. R. W. Govan Lysogeny and bacteriophage host range within the Burkholderia cepacia complex J. Med. Microbiol., June 1, 2003; 52(6): 483 - 490. [Abstract] [Full Text] [PDF]
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V. Punj, R. Sharma, O. Zaborina, and A. M. Chakrabarty Energy-Generating Enzymes of Burkholderia cepacia and Their Interactions with Macrophages J. Bacteriol., May 15, 2003; 185(10): 3167 - 3178. [Abstract] [Full Text] [PDF]
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V. Ramisse, J. Balandreau, F. Thibault, D. Vidal, G. Vergnaud, and P. Normand DNA-DNA hybridization study of Burkholderia species using genomic DNA macro-array analysis coupled to reverse genome probing Int J Syst Evol Microbiol, May 1, 2003; 53(3): 739 - 746. [Abstract] [Full Text] [PDF]
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M. Tomich, A. Griffith, C. A. Herfst, J. L. Burns, and C. D. Mohr Attenuated Virulence of a Burkholderia cepacia Type III Secretion Mutant in a Murine Model of Infection Infect. Immun., March 1, 2003; 71(3): 1405 - 1415. [Abstract] [Full Text] [PDF]
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J. W. Chung, E. Altman, T. J. Beveridge, and D. P. Speert Colonial Morphology of Burkholderia cepacia Complex Genomovar III: Implications in Exopolysaccharide Production, Pilus Expression, and Persistence in the Mouse Infect. Immun., February 1, 2003; 71(2): 904 - 909. [Abstract] [Full Text] [PDF]
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T. Coenye and J. J. LiPuma Population structure analysis of Burkholderia cepacia genomovar III: varying degrees of genetic recombination characterize major clonal complexes Microbiology, January 1, 2003; 149(1): 77 - 88. [Abstract] [Full Text] [PDF]
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M. D. Lefebre and M. A. Valvano Construction and Evaluation of Plasmid Vectors Optimized for Constitutive and Regulated Gene Expression in Burkholderia cepacia Complex Isolates Appl. Envir. Microbiol., December 1, 2002; 68(12): 5956 - 5964. [Abstract] [Full Text] [PDF]
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D. P. Speert, M. E. Campbell, D. A. Henry, R. Milner, F. Taha, A. Gravelle, A. G. F. Davidson, L. T. K. Wong, and E. Mahenthiralingam Epidemiology of Pseudomonas aeruginosa in Cystic Fibrosis in British Columbia, Canada Am. J. Respir. Crit. Care Med., October 1, 2002; 166(7): 988 - 993. [Abstract] [Full Text]
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S. C. M. Miller, J. J. LiPuma, and J. L. Parke Culture-Based and Non-Growth-Dependent Detection of the Burkholderia cepacia Complex in Soil Environments Appl. Envir. Microbiol., August 1, 2002; 68(8): 3750 - 3758. [Abstract] [Full Text] [PDF]
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E. MAHENTHIRALINGAM, A. BALDWIN, and P. VANDAMME Burkholderia cepacia complex infection in patients with cystic fibrosis J. Med. Microbiol., July 1, 2002; 51(7): 533 - 538. [Abstract] [Full Text] [PDF]
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T. Coenye, J. Goris, T. Spilker, P. Vandamme, and J. J. LiPuma Characterization of Unusual Bacteria Isolated from Respiratory Secretions of Cystic Fibrosis Patients and Description of Inquilinus limosus gen. nov., sp. nov. J. Clin. Microbiol., June 1, 2002; 40(6): 2062 - 2069. [Abstract] [Full Text] [PDF]
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S. Brisse, S. Stefani, J. Verhoef, A. Van Belkum, P. Vandamme, and W. Goessens Comparative Evaluation of the BD Phoenix and VITEK 2 Automated Instruments for Identification of Isolates of the Burkholderia cepacia Complex J. Clin. Microbiol., May 1, 2002; 40(5): 1743 - 1748. [Abstract] [Full Text] [PDF]
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J. F. Salles, F. A. De Souza, and J. D. van Elsas Molecular Method To Assess the Diversity of Burkholderia Species in Environmental Samples Appl. Envir. Microbiol., April 1, 2002; 68(4): 1595 - 1603. [Abstract] [Full Text] [PDF]
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