Proposal of Spirillum winogradskyi sp. nov., a novel microaerophilic species, an emended description of the genus Spirillum and Request for an Opinion regarding the status of the species Spirillum volutans Ehrenberg 1832, by D. Podkopaeva, M. Grabovich, J. Kuever, A. Lysenko, T. Tourova, T. Kolganova and G. Dubinina
International Journal of Systematic and Evolutionary Microbiology vol. 59, part 11, pp. 2916 - 2920
Supplementary Fig. S1. (a, b) Phase-contrast micrographs showing morphology of strain D-427T with coccoid bodies in the stationary growth phase (a) and the accumulation of elemental sulfur in cells grown on MPSS medium with FeS (b). Bar, 7 µm. (c, d) Transmission electron micrographs of strain D-427T showing sheathed flagella arranged in tufts (c) (whole-cell preparation stained with 1 % ammonium molybdate; bar, 1 µm) and the ultrastructure, with capsules of a polysaccharide nature stained with ruthenium red (d) (bar, 4 µm).
Supplementary Table S1. Cellular fatty acid composition of strain D-427T
Values are percentages of total fatty acids. a, Anteiso-branched; i, iso-branched.
| Fatty acid | Proportion (%) |
| 14:0 | 2.2 |
| i-15:0 | 0.1 |
| a-15:0 | 0.5 |
| 15:0 | 1.9 |
| 16:1 | 36.4 |
| 16:0 | 32.1 |
| a-17:0 | 0.6 |
| 17:1 | 2.5 |
| 17:0 | 1.8 |
| 18:1Δ11 | 17.9 |
| 18:0 | 3.8 |
| 20:0 | 0.2 |
