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Published online ahead of print on 6 November 2009 as doi:ijs.0.019273-0
Int J Syst Evol Microbiol (2009); DOI 10.1099/ijs.0.019273-0
© 2009 International Union of Microbiological Societies
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Methanoculleus hydrogenitrophicus sp. nov., a novel methanogenic archaeon isolated from Zoige Plateau in China

Jianqing Tian1, Yanfen Wang2 and Xiuzhu Dong1,3

1 State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences;
2 Graduate University, Chinese Academy of Sciences

3 E-mail: dongxz{at}im.ac.cn

An obligate anaerobic methanogenic archaeal strain HCT was isolated from Zoige wetland at Tibetan Plateau in China. The strain was isolated through constructing an artificial butyrate-degrading consortium of co-culturing with a syntrophic bacterium, Syntrophomonas erecta subsp. sporosyntropha. Strain HCT was irregular coccoid with the diameter of 0.8-2 µm and singly arranged and only utilized H2/CO2 for growth and methane production. Growth occurred at temperature 18-45 °C, and optimally around 37 °C. The pH for growth was 5.0-8.5, and optimal growth was around pH 6.6. The G+C content of the genomic DNA was 60.2 mol%. 16S rRNA gene sequence homology analysis indicated that strain HCT was affiliated to the Genus of Methanoculleus at sequence similarities of 94.8-97.2% with the existing members. However, strain HCT distinguished from the described Methanoculleus species by not using formate for growth and methane formation, nor requiring acetate as growth factor. Based on the phylogenetic analysis and phenotypic characteristics, a novel species Methanoculleus hydrogenitrophicus sp. nov. is proposed, with strain HCT as the type strain (=CGMCC1.5146T=JCM 16311T).






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