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Castellaniella daejeonensis sp. nov., isolated from soil

¹ Research and Development Division, H-Plus Eco Ltd., BVC #301, KRIBB, Eoeun-dong, Yuseong-gu, Daejeon;
² Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1, Guseon;
³ Pai Chai University

⁴ E-mail: l_ten{at}yahoo.com

A Gram-negative, non-spore-forming, motile, facultative anaerobic bacterial strain, designated MJ06T, was isolated from oil-contaminated soil and was characterized taxonomically by using a polyphasic approach. Strain MJ06T contained ubiquinone Q-8 as the major respiratory lipoquinone, putrescine as the predominant polyamine and phosphatidylethanolamine, diphosphatidylglycerol, and phosphatidylglycerol as major polar lipids. The G + C content of the genomic DNA was 66.2 mol%. The major fatty acids were summed feature 4 (C16 : 17c and/or iso-C15 : 0 2-OH; 32.5 %), C16:0 (22.8 %) and summed feature 7 (C18 : 17c and/or C18 : 19t and/or C18 : 112t; 14.9 %). Comparative 16S rRNA gene sequence analysis showed that strain MJ06T belonged to the family Alcaligenaceae, class Betaproteobacteria, and joined the evolutionary radiation enclosed by the genus Castellaniella. 16S rRNA gene sequence similarities between strain MJ06T and its phylogenetically closest relatives Castellaniella denitrificans DSM 11046T, Castellaniella defragrans DSM 12141T, Castellaniella ginsengisoli KCTC 22398T and Castellaniella caeni KCTC 12197T were 98.6, 98.3, 97.8 and 97.3 %, respectively. Levels of similarity between strain MJ06T and all other recognized species of the family Alcaligenaceae were below 95.0 %. Strain MJ06T exhibited relatively low levels of DNA-DNA relatedness values with respect to C. defragrans DSM 12141T (52 %), C. denitrificans DSM 11046T (31 %), C. ginsengisoli KCTC 22398T (18 %) and C. caeni KCTC 12197T (15 %). On the basis of its phenotypic and genotypic properties together with phylogenetic distinctiveness, strain MJ06T (=KCTC 22454T= JCM 16240T) should be classified in the genus Castellaniella as the type strain of a novel species, for which the name Castellaniella daejeonensis sp. nov. is proposed.