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Halopelagius inordinatus gen. nov., sp. nov., a new member of the family Halobacteriaceae isolated from a marine solar saltern

¹ School of food & biological engineering, Jiangsu University;
² Second Institute of Oceanography, State Oceanic Administration;
³ Institute of Microbiology, Chinese Academy of Sciences;
⁴ Institute of Life Sciences,The Hebrew University of Jerusalem

⁵ E-mail: cuihenglin{at}ujs.edu.cn

Two extremely halophilic archaea, strains RO5-2T and RO5-14, were isolated from Rudong marine solar saltern in Jiangsu, China. Cells of the two strains were pleomorphic, motile, and stained Gram-negative. Colonies were red-pigmented. Strains RO5-2T and RO5-14 were able to grow at 20-50 °C (optimum 37 °C), at 2.6-4.8 M NaCl (optimum 3.4-3.9 M NaCl), at 0.03-0.7 M MgCl2 (optimum 0.5 M MgCl2) and at pH 5.5-8.0 (optimum pH 6.5-7.0). Cells lyse in distilled water and minimal NaCl concentration to prevent cell lysis is 12 % (w/v). The major polar lipids of the two strains were PG (phosphatidylglycerol), PGP-Me (phosphatidylglycerol phosphate methyl ester) and two major glycolipids chromatographically identical to S-DGD-1 and DGD-1. The 16S rRNA gene sequence of strains RO5-2T and RO5-14 showed 93.4-93.8 % similarity to the closest cultivated relative, Halosarcina pallida. The DNA G+C content of strains RO5-2T and RO5-14 is 61.0 mol% and 59.9 mol%, respectively. The DNA relatedness between strains RO5-2T and RO5-14 was 86.0 %. The phenotypic, chemotaxonomic and phylogenetic properties suggest that strains RO5-2T and RO5-14 represent a novel species in a new genus within the family Halobacteriaceae, for which the name Halopelagius inordinatus gen. nov., sp. nov. is proposed. The type strain is RO5-2T (= CGMCC 1.7739T = JCM 15773T)