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Saccharopolyspora phatthalungensis sp. nov., isolated from rhizospheric soil of Hevea brasiliensis

¹ Department of Microbiology, Faculty of Science, Kasetsart University;
² Department of Genetics, Faculty of Science, Kasetsart University;
³ Kitasato Institute for Life Sciences, Kitasato University

⁴ E-mail: fscikkd{at}ku.ac.th

The taxonomic position of a rhizospheric soil isolate, designated SR8.15^T, was determined using a polyphasic approach. Phylogenetic analysis based on an almost-complete 16S rRNA gene sequence of the strain showed that it formed a well-seperated sub-branch within the radiation of the genus Saccharopolyspora. The highest levels of 16S rRNA gene sequence similarity were found between the strain SR8.15^T and Saccharopolyspora shandongensis CGMCC 4.3530^T (98.9 %) and S. spinosa DSM 44228^T (98.5 %). However, these strains shared low DNA-DNA relatedness with each other. The organism was found to have chemical characteristics consistent with its classification in the genus Saccharopolyspora. It contained meso-DAP as the diagnostic diamino acid. Whole cell hydrolysate contained arabinose and galactose. The diagnostic phospholipids were phosphatidylcholine, phosphatidylglycerol and phosphatidylinositol. Main menaquinone was MK-9(H₄). No mycolic acid was detected. The predominant cellular fatty acids was iso-C_{16 : 0}. The G+C content of the genomic DNA was 70.3 mol %. In addition, the strain SR8.15^T had a phenotypic profile that readily distinguished it from recognized representatives of the genus Saccharopolyspora. It is evident from the combined genotypic and phenotypic properties that strain SR8.15^T represented a novel species of the genus Saccharopolyspora. The proposed name is Saccharopolyspora phatthalungensis sp. nov. (type strain is strain SR8.15^T = TISTR 1921^T = BCC 35844^T = NRRL B-24798^T)