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Saccharomonospora marina sp. nov., isolated from an ocean sediment in the East China Sea

¹ School of Life Science, Xiamen University, P.O. Box 958, Xiamen 361005, P.R. China;
² Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, 650091, P. R. China

³ E-mail: wjli{at}ynu.edu.cn

A novel aerobic, Gram-positive actinomycete strain, designed XMU15T, was isolated from an ocean sediment collected from Zhaoan Bay in the East China Sea, and it was subjected to a polyphasic approach to determine its taxonomic position. The isolate grew optimally at 28°C and pH 7.0 with the presence of 3%(w/v) NaCl on ISP medium 2. Gelatin liquefaction, milk coagulation, and nitrate reduction are positive, and catalase, cellulose hydrolysis, hydrogen sulfide melanin production, starch hydrolysis, urease activity and oxidase are negative. The predominant menaquinone of the isolate was MK-9 (H4), and meso-DAP was its diagnostic amino acid in the cell wall. The phospholipids of the isolate comprised diphosphatidylglycerol (DPG), phosphoglycerides (PG), phosphatidylinositol (PI), and a minor amout of phosphatidyl ethanolamine (PE). The major fatty acids of the strain were iso-C16:0 (26.36 %), C17:16c (16.80 %), C15:0 (16.2 %), C16:0 (8.90 %), C17:18c (7.69 %) and iso-C16:1H (5.95 %). The G+C content of the genomic DNA was 68.1 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that the isolate fell within the evolutionary radiation encompassed by the genus Saccharomonospora, and it showed the highest 16S rRNA gene sequence similarity (96.7%) to S. xinjiangensis DSM 44391T. Based on the results of 16S rRNA gene sequence analysis and phenotypic and genotypic characterisation, strain XMU15T (=KCTC 19701T =CCTCC AA 209048T) represents a novel species of the genus Saccharomonospora, for which the name Saccharomonospora marina sp. nov., is proposed.