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Three new species of Dactylosporangium isolated from soil: Dactylosporangium luridum sp. nov., Dactylosporangium luteum sp. nov. and Dactylosporangium salmoneum sp. nov.

¹ National Academy of Agricultural Science;
² University of Newcastle upon Tyne

³ E-mail: m.goodfellow{at}ncl.ac.uk

Forty strains isolated from soil taken from a hay meadow were assigned to the genus Dactylosporangium on the basis of colonial properties. 16S rRNA gene sequencing showed that the isolates formed a group that was most closely related to the type strain of Dactylosporangium aurantiacum, but well separated from other Dactylosporangium type strains and from 'Dactylosporangium salmoneum' NRRL B-16294. Twelve out of thirteen representative isolates had identical 16S rRNA gene sequences and formed a subclade that was distinct from corresponding phyletic lines composed of the remaining isolate, strain BK63, the 'Dactylosporangium salmoneum' strain and each of the Dactylosporangium type strains. DNA:DNA relatedness data showed that representatives of the multimembered 16S rRNA subclade, isolate BK63 and the 'Dactylosporangium salmoneum' subclade formed distinct genomic species; all of these organisms had chemotaxonomic and morphological properties consistent with their classification in the genus Dactylosporangium. They were also distinguished from one another and from the Dactylosporangium type strains using a range of phenotypic properties. The combined genotypic and phenotypic data showed that isolate BK63, isolates BK51, BK53 and BK69, and strain NRRL B-16294 should be classified in the genus Dactylosporangium as novel species. The names proposed for these taxa are Dactylosporangium luridum sp. nov., Dactylosporangium luteum sp. nov. and Dactylosporangium salmoneum sp. nov.; the respective type strains of these species are BK63T(= DSM 45324T = KACC 20933T = NRRL B-24775T), BK51T (= DSM 45323T = KACC 20899T = NRRL B-24774T) and NRRL B-16294T (= ATCC 31222T = DSM 43910T = JCM 3272T = NBRC 14103T).