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Paenibacillus prosopidis sp. nov., isolated from the nodules of Prosopis farcta in Tunisia

¹ IRNASA-CSIC;
² Faculté des Sciences de Gabes, Gabes, Tunisia;
³ Universidad de Salamanca, Spain;
⁴ Facultad de Farmacia, Sevilla, Spain

⁵ E-mail: evp{at}usal.es

A bacterial strain designated PW21T was isolated from root nodules of Prosopis farcta in Tunisia. Phylogenetic analysis based on 16S rRNA gene sequence placed the isolate into the genus Paenibacillus being its closest relatives P. glycanilyticus DS-1T and P. castaneae Ch-32T with 96.9% identity values. DNA hybridization measurements showed values lower than 25% with respect to these two species. The isolate was a Gram-variable, motile and sporulating rod. Catalase was positive and oxidase was weak. Aesculin, CMC (carboxymethylcellulose), xylan and starch were hydrolysed but casein and gelatin did not. Acetoine production was weak and nitrate reduction was negative. Urease production was negative. Growth was supported by many carbohydrates and organic acids as carbon source. MK-7 was the predominant menaquinone and anteiso-C15:0 , iso-C16:0 and iso-C15:0 were the major fatty acids. Major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine phosphatidylglycerol, a glycolipid, six phospholipids, an unidentified lipid and two unknown aminophosphoglycolipids. Mesodiaminopimelic acid was not detected in peptidoglycan. The G+C content was 52.9 mol%. Phylogenetic, chemotaxonomic and phenotypic analyses showed that strain PW21T should be considered a new species of genus Paenibacillus, for which the name Paenibacillus prosopidis sp. nov. is proposed (type strain, LMG 25259T, CECT 7506T, DSM 22405T).