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Agrococcus terreus sp. nov. and Micrococcus terreus sp. nov., isolated from forest soil

Institute of Microbiology, Chinese Academy of Sciences

¹ E-mail: liusj{at}sun.im.ac.cn

Two bacterial strains, DNG5T and V3M1T, isolated from forest soil of Changbai Mountains in China, were characterized using a polyphasic approach. The analysis of their 16S rRNA gene sequences indicated that strain DNG5T and V3M1T were phylogenetically related to members of the genus Agrococcus (the similarities ranging from 96.0-98.4%) and Micrococcus (the similarities ranging from 96.7-98.0%), respectively, within the order Actinomycetales. Strains DNG5T and V3M1T were Gram-positive and strictly aerobic, formed yellow-pigment colonies on LB agar. Cells of strain DNG5T were short rod-shaped with size of 0.4-0.5 x 0.8-1.0 µm, non-motile. The strain DNG5T contained MK-10 and MK-11 as the major respiratory quinones and anteiso-C15:0 (40.8%), anteiso-C17: 0 (23.2%) and iso-C16: 0 (22.8%) as the major fatty acids. The diamino acid in the peptidoglycan of strain DNG5T was 2,4-diaminobutyric acid and the murein was of the acetyl type. Cells of strain V3M1T were cocci, with diameter range of 0.6-0.7 µm. Cell wall peptidoglycan of strain V3M1T contained amino acid residues of lysine, glutamic acid, alanine and glycine. The strain V3M1T contained MK-7, MK-7(H2), MK-8 and MK-8(H2) as respiratory quinones and anteiso-C15:0 (78.2%) and iso-C15: 0 (13.1%) as the major cellular fatty acids. The DNA G+C contents of strains DNG5T and V3M1T were 75.9 and 67.2 mol%, respectively. The DNA-DNA relatedness of strain DNG5T to Agrococcus jejuensis JCM 14256T, Agrococcus jenensis JCM 9950T, Agrococcus baldri JCM 12132T and Agrococcus citreus JCM 12398T were 58.3%, 43.9%, 36.1% and 54.1%, respectively. The DNA-DNA relatedness of strain V3M1T to Micrococcus luteus CGMCC 1.2299T, Micrococcus antarcticus CGMCC 1.2373T and Micrococcus lylae CGMCC 1.2300T were 57.5%, 45.4% and 39.0%, respectively. Combining phenotypic and genotypic traits, strain DNG5T represents a novel species of the genus Agrococcus, for which the name Agrococcus terreus is proposed, with DNG5T (=CGMCC 1.6960T = NBRC 104260T) as the type strain. The strain V3M1T represents a novel species of the genus Micrococcus, for which the name Micrococcus terreus is proposed, with V3M1T (=CGMCC 1.7054T = NBRC 104258T) as the type strain.