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Staphylococcus rostri sp. nov., a hemolytic bacterium isolated from the nose of healthy pigs

Institute of Veterinary Bacteriology, University of Berne

¹ E-mail: vincent.perreten{at}vbi.unibe.ch

Twenty coagulase-negative Staphylococcus strains displaying an -hemolysis (-hemolysin) on sheep blood agar were isolated from the nose of different pigs in Switzerland. The strains were Gram-positive, non-motile cocci, catalase-positive and coagulase-negative. Sequence analysis of 16S rDNA, sodA, rpoB, dnaJ, hsp60 and phylogenetic characteristics revealed that the strains showed the closest relatedness to Staphylococcus microti CCM 4903T and Staphylococcus muscae DSM 7068T. The strains can be differentiated from S. microti by the absence of mannose fermentation and arginine arylamidase and from S. muscae by the absence of β-glucuronidase activity and production of alkaline phosphatase. The type strain ARI 262T share 20.1% respectively 31.9% DNA similarity to S. microti CCM 4903T and S. muscae DSM 7068T by DNA-DNA hybridization. Fatty acid i-C15:0, ai-C15:0 and i-C17:0 were the most common fatty acids. Cell wall structure analysis revealed the peptidoglycan type A3 L-Lys-Gly2-L-Ser-Gly (type A11.3). The presence of teichoic acid was determined by sequencing the N-acetyl-β-D-mannosaminyltransferase gene tarA which is involved in the teichoic acid ribitol biosynthesis. Menaquinone 7 (MK-7) was the predominant respiratory quinone. The G+C contents of ARI 262T is 38.8%. The isolated strains represent a novel species of the genus Staphylococcus, S. rostri sp. nov. The strains used for the characterization of S. rostri sp. nov. are the type strain ARI 262T (= DSM 21968T = CCUG 57266T) and strain ARI 602 (= DSM 21969 = CCUG 57267).