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Streptomyces xinghaiensis sp. nov., isolated from marine sediment

¹ Department of Bioscience and Bioengineering, Dalian University of Technology, Linggong Road 2, Dalian 116024, PR China
² The Key Laboratory for Microbial Resources of the Ministry of Education, and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China
³ Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, PR China
⁴ Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, PR China
⁵ DSMZ – Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, 7b Inhoffenstraße, D-38124 Braunschweig, Germany
⁶ Department of Biological Science, Myongji University, Yongin 449-728, Republic of Korea

Correspondence
Feng-Wu Bai
fwbai{at}dlut.edu.cn

A novel actinomycete, strain S187^T, was isolated from a marine sediment sample collected from Xinghai Bay, Dalian, China. Growth occurred on ISP medium 2 containing 0–9 % NaCl and at pH 6.0–9.0 and 10–45 °C. The cell wall of strain S187^T contained the isomer LL-diaminopimelic acid as the diagnostic diamino acid. The predominant menaquinones were MK-9(H₆) (40.8 %), MK-9(H₈) (38.2 %) and MK-9(H₂) (8.8 %). The major fatty acids were iso-C_{16 : 0} (29.6 %), anteiso-C_{15 : 0} (14.0 %) and anteiso-C_{17 : 0} (11.6 %). Cells contained phosphatidylethanolamine, phosphatidylinositol, phosphatidylglycerol, phosphatidylinositol mannosides and one unknown phospholipid. The G+C content of the genomic DNA was 72.01 mol%. The 16S rRNA gene sequence of the isolate had similarities of 98.1 and 97.5 % with those of Streptomyces flavofuscus NRRL B-8036^T (=DSM 41426^T) and Streptomyces albiaxialis DSM 41799^T, respectively, showing that the novel strain should be assigned to the genus Streptomyces. DNA–DNA hybridizations with the two above-mentioned Streptomyces species showed 31.4 and 46.9 % relatedness, respectively. Moreover, the three strains differed in some physiological and biochemical properties. Thus, on the basis of phenotypic and genotypic analyses, it is proposed that strain S187^T represents a novel species of the genus Streptomyces, for which the name Streptomyces xinghaiensis sp. nov. is proposed; the type strain is S187^T (=NRRL B-24674^T=CCTCC AA 208049^T=KCTC 19546^T).

A scanning electron micrograph of strain S187^T, a phylogenetic tree based on 16S rRNA gene sequences of S187^T and related strains constructed using the maximum-parsimony method and antimicrobial activities of S187^T and closely related strains are available as supplementary material with the online version of this paper.