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Nocardioides caeni sp. nov., isolated from wastewater

¹ Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Republic of Korea
² Department of Microbiology, College of Medicine, Chung-Ang University, 221 Heukseok-dong, Seoul, Republic of Korea

Correspondence
Jung-Hoon Yoon
jhyoon{at}kribb.re.kr

A Gram-positive, non-motile, rod- or coccoid-shaped bacterial strain, designated MN8^T, was isolated from sludge of domestic wastewater in Korea, and its taxonomic position was investigated by use of a polyphasic study. Strain MN8^T grew optimally at pH 6.5–7.5 and 30 °C and in the presence of 0–0.5 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain MN8^T fell within the cluster comprising Nocardioides species, clustering with Nocardioides simplex KCTC 9106^T, Nocardioides aromaticivorans H-1^T, Nocardioides kongjuensis A2-4^T and Nocardioides nitrophenolicus NSP41^T, with which it shared 98.4–99.0 % 16S rRNA gene sequence similarity. The chemotaxonomic properties of strain MN8^T were consistent with those of the genus Nocardioides: the cell-wall peptidoglycan type was based on LL-2,6-diaminopimelic acid, the predominant menaquinone was MK-8(H₄) and the major fatty acids were iso-C_{16 : 0} and C_{18 : 1}9c. The DNA G+C content was 71.5 mol%. Strain MN8^T exhibited levels of DNA–DNA relatedness of 13–31 % to the type strains of phylogenetically related Nocardioides species and could be differentiated from these species based on differences in phenotypic characteristics. On the basis of the data presented, strain MN8^T is considered to represent a novel species of the genus Nocardioides, for which the name Nocardioides caeni sp. nov. is proposed. The type strain is MN8^T (=KCTC 19600^T=CCUG 57506^T).

An extended neighbour-joining tree based on 16S rRNA gene sequences showing the phylogenetic position of strain MN8^T among Nocardioides species and representatives of some other related taxa is available as supplementary material with the online version of this paper.