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Novel members of the family Micromonosporaceae, Rugosimonospora acidiphila gen. nov., sp. nov. and Rugosimonospora africana sp. nov.

Paolo Monciardini^1,2, Linda Cavaletti^1,, Anna Ranghetti¹, Peter Schumann³, Manfred Rohde⁴, Ruggiero Bamonte^1,, Margherita Sosio^1,2, Alessandra Mezzelani⁵ and Stefano Donadio²

¹ Vicuron Pharmaceuticals, 21040 Gerenzano, Italy
² KtedoGen, via Fantoli 16/15, 20138 Milano, Italy
³ DSMZ – Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, D-38124 Braunschweig, Germany
⁴ Helmholtz-Zentrum für Infektionsforschung GmbH, D-38124 Braunschweig, Germany
⁵ ITB-CNR, via Fantoli 16/15, 20138 Milano, Italy

Correspondence
Paolo Monciardini
paolo.monciardini{at}ktedogen.com

Two novel Gram-positive-staining, acidophilic strains were isolated from soil samples. Both show typical features of filamentous actinomycetes. On the basis of 16S rRNA gene sequence analysis, the strains are members of the family Micromonosporaceae. The two strains contain hydroxydiaminopimelic acid, glycine, alanine and glutamic acid in the peptidoglycan. Fatty acid profiles clearly differentiate the two strains: cyclohexyl C_{17 : 0}, i-C_{16 : 0} and ai-C_{17 : 0} are predominant in Delta1^T, while the major components for Delta3^T are ai-C_{17 : 0} and i-C_{16 : 0}. The two strains also differ in their major menaquinones, MK-9(H₈, H₄, H₆) for Delta1^T and MK-9(H₈, H₆) for Delta3^T, and in phospholipid patterns; Delta1^T displays phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, phosphatidylethanolamine, methyl phosphatidylethanolamine and an unknown aminophospholipid, while Delta3^T also contains minor amounts of several unknown phospholipids in addition to these phospholipids. The whole-cell sugars of both strains are galactose, arabinose and xylose. The G+C content of the DNA is 72.7 mol% for Delta1^T and 71.9 mol% for Delta3^T. On the basis of chemotaxonomic, physiological and phylogenetic data, we propose Rugosimonospora gen. nov. to accommodate the two strains, with the description of Rugosimonospora acidiphila gen. nov., sp. nov. (the type species; type strain Delta1^T =DSM 45227^T =NBRC 104874^T) and Rugosimonospora africana sp. nov. (type strain Delta3^T =DSM 45228^T =NBRC 104875^T).

Present address: Bayer S.p.A., 20024 Garbagnate, Italy.

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains Delta1^T and Delta3^T are FM208261 and FM208262, respectively.

Signature nucleotides within the 16S rRNA gene sequence are detailed in a table available as supplementary material with the online version of this paper.