HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplementary Table Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Google Scholar Articles by Steven, B. Articles by Niederberger, T. D. PubMed PubMed Citation Articles by Steven, B. Articles by Niederberger, T. D. Agricola Articles by Steven, B. Articles by Niederberger, T. D.

Tumebacillus permanentifrigoris gen. nov., sp. nov., an aerobic, spore-forming bacterium isolated from Canadian high Arctic permafrost

¹ Department of Natural Resource Sciences, McGill University, Ste-Anne de Bellevue, Quebec, Canada
² Biotechnology Research Institute, National Research Council of Canada, Montreal, Quebec, Canada

Correspondence
Lyle G. Whyte
lyle.whyte{at}mcgill.ca

A Gram-positive, aerobic, rod-shaped bacterium (strain Eur1 9.5^T) was isolated from a 9-m-deep permafrost sample from the Canadian high Arctic. Strain Eur1 9.5^T could not be cultivated in liquid medium and grew over the temperature range 5–37 °C; no growth was observed at 42 °C and only slow growth was observed at 5 °C following 1 month of incubation. Eur1 9.5^T grew over the pH range 5.5–8.9 and tolerated NaCl concentrations of 0–0.5 % (w/v). Eur1 9.5^T grew heterotrophically on complex carbon substrates and chemolithoautotrophically on inorganic sulfur compounds, as demonstrated by growth on sodium thiosulfate and sulfite as sole electron donors. Eur1 9.5^T contained iso-C_{15 : 0} as the major cellular fatty acid and menaquinone 7 (MK-7) as the major respiratory quinone. The cell-wall peptidoglycan was of type A1. The DNA G+C content was 53.1 mol%. The 16S rRNA gene sequence of strain Eur1 9.5^T was only distantly related (87 % sequence similarity over 1407 bp) to any recognized bacterial species. Based on physiological and phylogenetic analyses, strain Eur1 9.5^T is suggested to represent a novel species of a new genus, for which the name Tumebacillus permanentifrigoris gen. nov., sp. nov. is proposed. The type strain of Tumebacillus permanentifrigoris is Eur1 9.5^T (=DSM 18773^T =JCM 14557^T).

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of Eur1 9.5^T is DQ444975.

A table detailing the cellular fatty acid composition of strain Eur1 9.5^T is available as supplementary material with the online version of this paper.