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Adlercreutzia equolifaciens gen. nov., sp. nov., an equol-producing bacterium isolated from human faeces, and emended description of the genus Eggerthella

¹ Fujicco Co. Ltd, 6-13-4 Minatojima-nakamachi, Chuo-ku, Kobe, Hyogo 650-8558, Japan
² Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan

Correspondence
Toshinari Maruo
t-maruo{at}fujicco.co.jp

Nine strains capable of metabolizing isoflavones to equol were isolated from human faeces. Four of the strains were characterized by determining phenotypic and biochemical features and their phylogenetic position based on 16S rRNA gene sequence analysis. These strains were related to Eggerthella sinensis HKU14^T with about 93 % 16S rRNA gene sequence similarity; they were asaccharolytic, obligately anaerobic, non-spore-forming, non-motile and Gram-positive coccobacilli. In enzyme activity tests, arginine dihydrolase, arginine and leucine arylamidases were positive but nitrate reduction, urease and β-glucosidase were negative. The major menaquinone was DMMK-6 (dimethylmenaquinone-6), while that of members of the genus Eggerthella was MMK-6 (methylmenaquinone-6). Furthermore, the cell-wall peptidoglycan type of these strains was A1, while that of members of the genus Eggerthella was A4. On the basis of these data, a new genus, Adlercreutzia gen. nov., is proposed with one species, Adlercreutzia equolifaciens sp. nov. The type strain of Adlercreutzia equolifaciens is FJC-B9^T (=JCM 14793^T =DSM 19450^T =CCUG 54925^T).

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains FJC-A10, FJC-B9^T, FJC-B20 and FJC-D53 are AB306660–AB306663.

Detailed menaquinone and fatty acid profiles of representative strains and related type strains and minimum-evolution and maximum-parsimony 16S rRNA gene sequence-based trees are available as supplementary material with the online version of this paper.