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Jiangella alkaliphila sp. nov., an actinobacterium isolated from a cave

Department of Science Education, Cheju National University, Jeju 690-756, Republic of Korea

Correspondence
Soon Dong Lee
sdlee{at}cheju.ac.kr

A mycelium-forming actinomycete, designated strain D8-87^T, was isolated from a soil collected from a cave in Jeju, Republic of Korea. Comparative 16S rRNA gene sequence analysis showed that the organism was closely related to the type and only species of the genus Jiangella. The level of 16S rRNA gene sequence similarity between strain D8-87^T and the type strain of Jiangella gansuensis was 99.2 %. The novel organism produced extensively branched aerial and substrate mycelia, which fragmented into short or elongated rod-shaped cells. Whole-cell hydrolysates of the isolate contained LL-diaminopimelic acid, glucose, mannose and arabinose. The predominant menaquinone was MK-9(H₄). The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylinositol, phosphatidylinositol mannoside and an unknown phospholipid. The predominant fatty acids were anteiso-C_{15 : 0}, iso-C_{16 : 0} and iso-C_{14 : 0}. The DNA G+C content of strain D8-87^T was 71.5 mol%. Levels of DNA–DNA relatedness between strain D8-87^T and J. gansuensis KCTC 19044^T were 23.4 and 34.6 %. On the basis of the phenotypic and DNA–DNA hybridization data presented, strain D8-87^T is considered to represent a novel species of the genus Jiangella, for which the name Jiangella alkaliphila is proposed. The type strain is D8-87^T (=JBRI 2008^T=KCTC 19222^T=DSM 45079^T).

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain D8-87^T is AM422451.

A scanning electron micrograph of cells of strain D8-87^T and a neighbour-joining tree showing the phylogenetic relationships of strain D8-87^T within the radiation of the order Actinomycetales based on 16S rRNA gene sequences are available with the online version of this paper.