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Chryseobacterium bovis sp. nov., isolated from raw cow's milk

¹ Department of Evolutionary and Environmental Biology, Faculty of Science and Science Education, University of Haifa, Mount Carmel, Haifa 31905, Israel
² Department of Biology Education, Faculty of Science and Science Education, University of Haifa, Oranim, Tivon 36006, Israel

Correspondence
Malka Halpern
mhalpern{at}research.haifa.ac.il

Three Gram-negative, rod-shaped, oxidase-positive, aerobic, non-motile bacterial strains, designated H9^T, H10 and H15, were isolated during a study on the diversity of culturable psychrotolerant bacteria in raw cow's milk. Comparisons of 16S rRNA gene sequences showed that the three strains were very closely related to each other (sequence similarities of 99.6–99.8 %). A polyphasic taxonomic study of the isolates resulted in their identification as members of the genus Chryseobacterium (family Flavobacteriaceae, phylum Bacteroidetes). The three strains showed 96.9 % sequence similarity with respect to the type strains of described Chryseobacterium species, indicating that H9^T, H10 and H15 represent a novel species of the genus Chryseobacterium. The three strains grew at 7–37 °C (strain H10 grew at up to 41 °C), with 0–2.5 % NaCl and at pH 5.0–9.8. The dominant cellular fatty acids of strain H9^T were 15 : 0 iso (38.9 %), 15 : 0 anteiso (15.6 %) and 17 : 0 iso 3-OH (12.7 %). Strain H10 also possessed 17 : 1 iso 9c (14.8 %) as a major fatty acid. On the basis of phenotypic properties and phylogenetic distinctiveness, the three milk isolates represent a novel species in the genus Chryseobacterium, for which the name Chryseobacterium bovis sp. nov. is proposed. The type strain is H9^T (=LMG 24227^T =DSM 19482^T).

An electron micrograph of strain H9^T is available as a supplementary figure with the online version of this paper.