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Nocardiopsis quinghaiensis sp. nov., isolated from saline soil in China

Yi-Guang Chen^1,2,, Xiao-Long Cui^1,, Reiner M. Kroppenstedt³, Erko Stackebrandt³, Meng-Liang Wen¹, Li-Hua Xu¹ and Cheng-Lin Jiang¹

¹ Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, People's Republic of China
² College of Bio-resources and Environmental Science, Jishou University, Jishou, Hunan 416000, People's Republic of China
³ DSMZ – Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Inhoffenstraße 7b, D-38124 Braunschweig, Germany

Correspondence
Reiner M. Kroppenstedt
kdt{at}dsmz.de

A previously unknown Gram-positive, obligately aerobic actinomycete, YIM 28A4^T, was isolated from a sample of saline soil collected from the Qaidam Basin in Qinghai Province, north-west China, and was investigated using a polyphasic taxonomic approach. The strain grew well on most of the media tested, producing white to pale-yellow substrate mycelium, white aerial mycelium and straight to flexuous hyphae. The substrate mycelium was well developed and fragmented with age; the aerial mycelium produced long, straight spore chains. The spore chains were composed of non-motile, smooth-surfaced, rod-shaped spores. No diffusible pigments were produced on any of the media tested. The strain grew in the presence of 0–10 % (w/v) NaCl and at pH 6.0–8.0, with optimum growth occurring at 3 % (w/v) NaCl and pH 7.0. It grew at 10–37 °C, the optimum growth temperature being 28 °C. Whole-cell hydrolysates of strain YIM 28A4^T contained meso-diaminopimelic acid and no diagnostic sugars. The predominant phospholipids were phosphatidylcholine, phosphatidylglycerol and diphosphatidylglycerol. The predominant menaquinones were MK-10, MK-10(H₂), MK-11 and MK-11(H₂). The major cellular fatty acids were iso-C_{16 : 0}, anteiso-C_{15 : 0} and anteiso-C_{17 : 0}. The DNA G+C content was 67.1 mol%. The morphological and chemotaxonomic characteristics of the isolate matched those described for Nocardiopsis species. A phylogenetic analysis based on 16S rRNA gene sequence comparisons confirmed that strain YIM 28A4^T was a member of the genus Nocardiopsis and most closely related to the type strains Nocardiopsis aegyptia DSM 44442^T and Nocardiopsis halotolerans DSM 44410^T, showing 98.1 and 97.8 % 16S rRNA gene sequence similarity, respectively. Strain YIM 28A4^T can be differentiated from these type strains by using phenotypic, phylogenetic and DNA–DNA hybridization data. On the basis of the polyphasic evidence, strain YIM 28A4^T represents a novel species of the genus Nocardiopsis, for which the name Nocardiopsis quinghaiensis sp. nov. is proposed. The type strain is YIM 28A4^T (=DSM 44739^T =CGMCC 4.3494^T).

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain YIM 28A4^T is EF597511.