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Int J Syst Evol Microbiol 58 (2008), 505-509; DOI  10.1099/ijs.0.65332-0
© 2008 International Union of Microbiological Societies

Bacillus butanolivorans sp. nov., a species with industrial application for the remediation of n-butanol

Nomeda Kuisiene1, Juozas Raugalas1, Cathrin Spröer2, Reiner M. Kroppenstedt2 and Donaldas Chitavichius1

1 Department of Plant Physiology and Microbiology, Vilnius University, Chiurlionio 21/27, Vilnius LT-03101, Lithuania
2 DSMZ – Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Inhoffenstraße 7b, 38124 Braunschweig, Germany

Correspondence
Nomeda Kuisiene
nomeda.kuisiene{at}gf.vu.lt

Four bacterial strains, designated K9T, K105, K1012A and K101, were isolated from soil in Lithuania. All these strains could use n-butanol as a sole carbon source. The strains grew in a medium containing 12–120 mM n-butanol. The strains were strictly aerobic, Gram-positive endospore-formers. The best growth was achieved at 25 °C and pH 7.0 in medium containing 1 % (w/v) NaCl. The strains showed identical profiles of 16S–23S rRNA internal transcribed spacer PCR and nearly identical 16S rRNA gene PCR-RFLP electrophoretic patterns and physiological characteristics, demonstrating their relationship at the species level. The cellular fatty acid profile of K9T consisted of significant amounts of the C15 branched-chain fatty acids iso-C15 : 0 (16.78 %) and anteiso-C15 : 0 (45.80 %). The diagnostic cell-wall diamino acid was meso-diaminopimelic acid. The 16S rRNA gene sequence of K9T showed the highest similarity to the sequences of Bacillus simplex DSM 1321T and Bacillus muralis LMG 20238T (98.3 and 97.7 %, respectively). The DNA G+C content was 37.4 mol%. Studies of DNA–DNA relatedness, morphological, physiological and chemotaxonomic analyses and phylogenetic data based on 16S rRNA gene sequencing allowed strains K9T, K105, K1012A and K101 to be described as members of a novel species of the genus Bacillus, for which the name Bacillus butanolivorans sp. nov. is proposed. The type strain is K9T (=DSM 18926T =LMG 23974T).

Abbreviations: ARDRA, amplified rDNA restriction analysis; RISA, ribosomal intergenic spacer analysis

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain K9T is EF206294.

ARDRA and RISA profiles for the novel strains are available as supplementary material with the online version of this paper.






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