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Haloterrigena hispanica sp. nov., an extremely halophilic archaeon from Fuente de Piedra, southern Spain

¹ Istituto di Chimica Biomolecolare, Comprensorio ex Olivetti, via Campi Flegrei 34, 80078 Pozzuoli, Napoli, Italy
² CSIC, Estacion Experimental del Zaidin, Department of Earth Sciences and Environmental Chemistry, Profesor Albareda 1, 18008 Granada, Spain
³ Istituto di Ricerche di Biologia Molecolare Angeletti IRBM, Pomezia, Roma, Italy

Correspondence
Barbara Nicolaus
bnicolaus{at}icmib.na.cnr.it

An extremely halophilic archaeon belonging to the order Halobacteriales was isolated from Fuente de Piedra salt lake, Spain. This strain, designated FP1^T, was a pleomorphic coccoid, neutrophilic and required at least 15 % (w/v) NaCl for growth. Strain FP1^T grew at 37–60 °C, with optimal growth at 50 °C. Mg²⁺ was not required, but growth was observed with up to 10 % (w/v) MgSO₄. Polar lipid analysis revealed the presence of mannose-6-sulfate(1-2)-glucose glycerol diether as a major glycolipid. Both C₂₀C₂₀ and C₂₀C₂₅ core lipids were present. The genomic DNA G+C content was 62.0 mol%. Phylogenetic analysis based on comparison of 16S rRNA gene sequences demonstrated that the isolate was most closely related to species of the genus Haloterrigena. DNA–DNA reassociation values between strain FP1^T and the most closely related species of the genus Haloterrigena (Haloterrigena thermotolerans, Haloterrigena saccharevitans and Haloterrigena limicola) were lower than 29 %. It is therefore considered that strain FP1^T represents a novel species of the genus Haloterrigena, for which the name Haloterrigena hispanica sp. nov. is proposed. The type strain is FP1^T (=DSM 18328^T=ATCC BAA-1310^T).

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain FP1^T is AM285297.

A thin-layer chromatogram of total polar lipids and a table giving ¹³C NMR assignments for polar lipids from strain FP1^T and related species are available with the online version of this paper.

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