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Microcella alkaliphila sp. nov., a novel member of the family Microbacteriaceae isolated from a non-saline alkaline groundwater, and emended description of the genus Microcella

¹ Departamento de Zoologia, Universidade de Coimbra, 3004-517 Coimbra, Portugal
² Centro de Neurociências de Biologia Celular, Universidade de Coimbra, 3004-517 Coimbra, Portugal
³ Departamento de Bioquímica, Universidade de Coimbra, 3004-517 Coimbra, Portugal
⁴ Instituto do Ambiente e Vida, Universidade de Coimbra, 3004-517 Coimbra, Portugal

Correspondence
António Veríssimo
averiss{at}ci.uc.pt

A high-G+C-content Gram-positive bacterium, designated as strain AC4r^T, was isolated from a highly alkaline, non-saline groundwater environment (pH 11.4). This organism formed small rod-shaped cells, was aerobic, heterotrophic, catalase-positive and oxidase-negative and had an optimum growth temperature of 35 °C and an optimum pH of 9.5. The strain possessed a B2-type cell-wall peptidoglycan, with D-Orn as the diagnostic diamino acid. The major respiratory quinones were unsaturated menaquinones with 13 and 14 isoprene units. The predominant fatty acids were anteiso-15 : 0, iso-16 : 0, iso-14 : 0 and iso-15 : 0. The G+C content of the DNA was 67.1 mol%. In a 16S rRNA gene sequence analysis, strain AC4r^T showed the highest level of similarity (99.2 %) to the type strain of Microcella putealis; however, the DNA–DNA reassociation value between these two organisms was low (38.3 %). On the basis of phylogenetic analysis, the DNA–DNA reassociation value and distinct phenotypic characteristics, strain AC4r^T represents a novel species within the genus Microcella, for which the name Microcella alkaliphila sp. nov. is proposed. The type strain is AC4r^T (=LMG 22690^T=CIP 108473^T).

The fatty acid compositions of strain AC4r^T and Microcella putealis CV2^T and a phylogenetic dendrogram are available as supplementary material in IJSEM Online.

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