HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Ma, K. Articles by Dong, X. Search for Related Content PubMed PubMed Citation Articles by Ma, K. Articles by Dong, X. Agricola Articles by Ma, K. Articles by Dong, X.

Methanosaeta harundinacea sp. nov., a novel acetate-scavenging methanogen isolated from a UASB reactor

¹ State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, P. R. China
² Graduate School, Chinese Academy of Sciences, Beijing 100049, P. R. China

Correspondence
Xiuzhu Dong
dongxz{at}sun.im.ac.cn

Two methanogenic strains, 8Ac^T and 6Ac, were isolated from an upflow anaerobic sludge blanket reactor treating beer-manufacture wastewater in Beijing, China. Cells of strains 8Ac^T and 6Ac were rod-shaped (0·8–1·0x3–5 µm) and non-motile, occurring singly or in pairs; however, at high cell density the cells were arranged in long chains within a common sheath. The two strains used acetate exclusively for growth and methane production. The specific growth rate of strain 8Ac^T was 0·030 h^–1 when growing in acetate (20 mM) at 37 °C. The temperature range for growth was 25–45 °C, with the fastest growth at 34–37 °C. The pH range for growth and methane production was 6·5–9·0, with the fastest growth at pH 7·2–7·6. The G+C content of genomic DNA of strain 8Ac^T was 55·7 mol%. Phylogenetic analysis based on 16S rRNA gene sequence similarity showed that the novel strains clustered with Methanosaeta species; the 16S rRNA gene sequence similarities between strain 8Ac^T and Methanosaeta concilii DSM 3013 and ‘Methanosaeta thermophila’ DSM 6194 were 92·5 and 87·3 %, respectively. The sequence similarity levels of mcrA, the gene encoding the -subunit of methyl-coenzyme M reductase, and of the deduced amino acids of mcrA, between strain 8Ac^T and Methanosaeta concilii DSM 3671^T were 36 and 78·9 %, respectively. Based on the phylogenetic and phenotypic analyses, the novel species Methanosaeta harundinacea sp. nov. is proposed, with strain 8Ac^T (=JCM 13211^T=CGMCC 1.5026^T) as the type strain.

Published online ahead of print on 9 September 2005 as DOI 10.1099/ijs.0.63887-0.

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of Methanosaeta harundinacea 8Ac^T and 6Ac are AY817738 and AY970347, respectively, and those for the partial sequences of mcrA of Methanosaeta harundinacea 8Ac^T and 6Ac are AY970348 and AY970349, respectively.