IJSEM Journal of Clinical Microbiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lee, I.-M.
Right arrow Articles by Seemüller, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lee, I.-M.
Right arrow Articles by Seemüller, E.
Agricola
Right arrow Articles by Lee, I.-M.
Right arrow Articles by Seemüller, E.
Int J Syst Evol Microbiol 54 (2004), 1037-1048; DOI  10.1099/ijs.0.02843-0
© 2004 International Union of Microbiological Societies

‘Candidatus Phytoplasma asteris’, a novel phytoplasma taxon associated with aster yellows and related diseases

I.-M. Lee1, D. E. Gundersen-Rindal2, R. E. Davis1, K. D. Bottner1, C. Marcone3 and E. Seemüller4

1 USDA-ARS Molecular Plant Pathology Laboratory, Beltsville, MD 20705, USA
2 Insect Biocontrol Laboratory, Beltsville, MD 20705, USA
3 Dept Biologia, Difesa e Biotecnologie Agro-Forestali, University of Basilicata, 85100 Potenza, Italy
4 Biologische Bundesanstalt, Institut für Pflanzenschutz im Obstbau, D-69221 Dossenheim, Germany

Correspondence
I.-M. Lee
leeim{at}ba.ars.usda.gov

Aster yellows (AY) group (16SrI) phytoplasmas are associated with over 100 economically important diseases worldwide and represent the most diverse and widespread phytoplasma group. Strains that belong to the AY group form a phylogenetically discrete subclade within the phytoplasma clade and are related most closely to the stolbur phytoplasma subclade, based on analysis of 16S rRNA gene sequences. AY subclade strains are related more closely to their culturable relatives, Acholeplasma spp., than any other phytoplasmas known. Within the AY subclade, six distinct phylogenetic lineages were revealed. Congruent phylogenies obtained by analyses of tuf gene and ribosomal protein (rp) operon gene sequences further resolved the diversity among AY group phytoplasmas. Distinct phylogenetic lineages were identified by RFLP analysis of 16S rRNA, tuf or rp gene sequences. Ten subgroups were differentiated, based on analysis of rp gene sequences. It is proposed that AY group phytoplasmas represent at least one novel taxon. Strain OAY, which is a member of subgroups 16SrI-B, rpI-B and tufI-B and is associated with evening primrose (Oenothera hookeri) virescence in Michigan, USA, was selected as the reference strain for the novel taxon ‘Candidatus Phytoplasma asteris’. A comprehensive database of diverse AY phytoplasma strains and their geographical distribution is presented.

Abbreviations: AY, aster yellows

The GenBank/EMBL/DDBJ accession numbers for the aster yellows phytoplasma strain OAY sequences are M30790 (16S rDNA) and M74770 (rpl22–rps3).






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 2004 by the International Union of Microbiological Societies.