International Journal of Systematic and Evolutionary Microbiology, Vol 51, 327-337, Copyright © 2001 by Society for General Microbiology

Dethiosulfovibrio russensis sp. nov., Dethiosulfovibrio marinus sp. nov. and Dethiosulfovibrio acidaminovorans sp. nov., novel anaerobic, thiosulfate- and sulfur-reducing bacteria isolated from 'Thiodendron' sulfur mats in different saline environments

AV Surkov, GA Dubinina, AM Lysenko, FO Glockner and J Kuever
Laboratory of Ecology and Geochemical Activity of Microorganisms, Institute of Microbiology of Russian Academy of Sciences, Moscow, Russia

Four strains of strictly anaerobic, sulfur- and thiosulfate-reducing bacteria, SR12(T), SR13, SR15(T) and WS100(T), were isolated from 'Thiodendron' sulfur mats obtained from different saline environments. All isolates were motile, Gram-negative, non-spore-forming curved rods with pointed or rounded ends. The sizes of cells varied from 0.9 x 3--5 microm for strains SR12(T), SR13 and SR15(T) to 0.9 x 4--8 microm for strain WS100(T). All strains could form long spiral filamentous cells up to 70--110 microm during the early stage of growth. All strains were motile by a tumbling movement and possessed lateral flagella arranged at the concave side of cells. Incomplete cross-septa were distinctive features of all strains. Growth occurred at temperatures of 10--40 degrees C with an optimum at 28 degrees C. The pH limits for growth were 5.5 to 8.0, with optimal growth at pH 6.5--7.0. All isolates were obligately anaerobic and slightly halophilic and grew in media containing 0.5--5% NaCl with an optimum at 2% NaCl. All strains were chemoorganoheterotrophic, having a fermentative type of metabolism and utilized proteins, peptides, amino acids and some organic acids, but not sugars, fatty acids or alcohols. Some organic substrates (isoleucine, valine, alanine, glutamate) were utilized only by strain SR12(T) in the presence of sulfur or thiosulfate. Fermentation of citrate yielded mainly acetate, CO(2) and H(2). Sulfur and thiosulfate were reduced to hydrogen sulfide during the fermentation of organic substances, which increased cell yields and growth rates. Sulfate, sulfite, fumarate, nitrate, Fe(2)O(3), MnO(2), DMSO and elemental selenium were not used as electron acceptors by these strains. The G+C contents of the DNA were 51 mol% for strains SR12(T), SR13 and SR15(T) and 52 mol% for strain WS100(T). Based on morphological, physiological and phylogenetic similarities, all four isolates could be assigned to three new species of the genus Dethiosulfovibrio, named Dethiosulfovibrio russensis (type strain DSM 12538(T)), Dethiosulfovibrio marinus (type strain DSM 12537(T)) and Dethiosulfovibrio acidaminovorans (type strain DSM 12590(T)).

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