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International Journal of Systematic and Evolutionary Microbiology, Vol 50, 2069-2073, Copyright © 2000 by Society for General Microbiology

Extended method for discrimination of Aeromonas spp. by 16S rDNA RFLP analysis

M Figueras, L Soler, MR Chacon, J Guarro and AJ Martinez-Murcia
Departamento de Ciencias Medicas Basicas, Facultad de Medicina y Ciencias de la Salud, Universidad Rovira y Virgili, San Lorenzo 21, 43201 Reus, Spain

A previously described molecular method, based on 16S rDNA RFLP analysis, for the identification of Aeromonas spp. was unable to separate the species Aeromonas salmonicida, Aeromonas bestiarum and the recently described Aeromonas popoffii. In this study, the method has been extended with endonucleases AlwNI and PstI for the identification of these species. A molecular frame for the identification of all known Aeromonas spp. is presented.


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