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Pyrococcus glycovorans sp. nov., a hyperthermophilic archaeon isolated from the East Pacific Rise

Georges Barbier¹, Anne Godfroy¹, Jean-Roch Meunier^2,, Joël Quérellou¹, Marie-Anne Cambon¹, Françoise Lesongeur¹, Patrick A. D. Grimont² and Gérard Raguénès¹

¹ Laboratoire de Caractérisation des Microorganismes Marins, IFREMER, Centre de Brest, BP 70, 29280 Plouzané, France
² Unité des Entérobactéries, Institut Pasteur, 75724 Paris cedex 15, France

Author for correspondence: Georges Barbier. Tel: +33 2 98 22 45 21. Fax: +33 2 98 22 45 45. e-mail: gbarbier{at}ifremer.fr

ABSTRACT

A hyperthermophilic archaeon, strain AL585^T, was isolated from a deep-sea hydrothermal vent located on the East Pacific Rise at latitude 13 ° N and a depth of 2650 m. The isolate was a strictly anaerobic coccus with a mean cell diameter of 1 µm. The optimum temperature, pH and concentration of sea salt for growth were 95 °C, 7·5 and 30 g l-¹. Under these conditions, the doubling time and cell yield were 0·5 h and 5 x 10⁸ cells ml-¹. Strain AL585^T grew preferentially in media containing complex proteinaceous carbon sources, glucose and elemental sulfur. The G+C content of the DNA was 47 mol%. Sequencing of the 16S rDNA gene showed that strain AL585^T belonged to the genus Pyrococcus and was probably a new species. This was confirmed by total DNA hybridization. Consequently, this strain is described as a new species, Pyrococcus glycovorans sp. nov.

Key Words: archaea • Pyrococcus • hyperthermophile • deep-sea • hydrothermal vent

Present address: L'Oréal, Recherche avancée, DCSP - Phototoxicité, 1 Avenue Eugéne Schueller, 93600 Aulnay-sous-Bois, France.

The GenBank/EMBL accession number for the 16S rDNA sequence of strain AL585^T is Z70247.