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Int J Syst Bacteriol 46 (1996), 967-971; DOI 10.1099/00207713-46-4-967
© 1996 Society for General Microbiology
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Leucobacter komagatae gen. nov., sp. nov., a New Aerobic Gram-Positive, Nonsporulating Rod with 2,4-Diaminobutyric Acid in the Cell Wall

MARIKO TAKEUCHI1,*, NORBERT WEISS2, PETER SCHUMANN3 and AKIRA YOKOTA1,{dagger}

1Institute for Fermentation, Osaka, Yodogawa-ku, Osaka 532, Japan
2DSM-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, D-07745 Braunschweig, Germany
3DSM-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Jena, Germany

* Corresponding author. Mailing address: Institute for Fermentation, Osaka, 17-85, Juso-honmachi 2-chome, Yodogawa-ku, Osaka 532, Japan. Phone: 06-300-6555. Fax: 06-300-6814.

ABSTRACT

A new aerobic, gram-positive, nonsporulating rod-shaped organism is described. Strain IFO 15245T (T = type strain) has the following characteristics: The menaquinone contains a side chain with 11 isoprenyl units; the guanine-plus-cytosine content of the DNA is 66.2 moI%; 2,4-diaminobutyric acid, glutamic acid, alanine, glycine, and {gamma} -aminobutyric acid are present in the cell wall at a molar ratio of ca. 1:1:2:1:1; and glucose and galactose are also present in the cell wall. A comparison of partial 16S rRNA sequences revealed that IFO 15245T represents a distinct line of descent within the gram-positive bacteria with high guanine-plus-cytosine contents. The taxonomic characteristics of this organism are different from those of previously described aerobic, gram-positive, nonsporulating, rod-shaped bacteria. The name Leucobacter komagatae gen. nov., sp. nov., is proposed for this organism. The type strain is strain IFO 15245.


{dagger} Present address: Institute of Molecular and Cellular Biosciences, The University of Tokyo, Yayoi, Bunkyo-ku, Tokyo 113, Japan.




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