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Int J Syst Bacteriol 46 (1996), 782-791; DOI 10.1099/00207713-46-3-782
© 1996 Society for General Microbiology
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Polyphasic Analysis of Strains of the Genus Capnocytophaga and Centers for Disease Control Group DF-3

P. VANDAMME1,2,*, M. VANCANNEYT1, A. VAN BELKUM3, P. SEGERS1, W. G. V. QUINT4, K. KERSTERS1, B. J. PASTER5 and F. E. DEWHIRST5

1Laboratory of Microbiology, University of Ghent, Ghent
2Department of Microbiology, University Hospital Antwerp UIA, Antwerp, Belgium
3Department of Bacteriology, University Hospital Rotterdam, Rotterdam
4Department of Molecular Biology, Diagnostic Center SSDZ, Delft, The Netherlands
5Department of Molecular Genetics, Forsyth Dental Center, Boston, Massachusetts 02115

* Corresponding author. Mailing address: Laboratorium voor Microbiologie, Ledeganckstraat 35, B-9000 Ghent, Belgium. Phone: (32) 9.264.51.14. Fax: (32) 9.264.53.46. Electronic mail address: Peter.Vandamme{at}rug.ac.be.

ABSTRACT

A polyphasic approach was used to determine the relationships between well-characterized reference strains representing all seven Capnocytophaga species. One Centers for Disease Control (CDC) group DF-3 strain, a presumed relative of the genus Capnocytophaga, and 15 field isolates were included as well. Fourteen isolates were assigned to named Capnocytophaga species, all of which could be differentiated by means of whole-organism protein electrophoresis. A separate position was occupied by the CDC group DF-3 strain and by one field isolate representing a novel Capnocytophaga species. The phylogenetic position of each taxon was determined by means of 16S rRNA sequence analysis. A considerable genotypic heterogeneity within the genus Capnocytophaga was detected in spite of the minimal phenotypic differences. Comparative 16S rRNA sequence analysis revealed that CDC group DF-3 is not a close relative of the capnocytophagas but constitutes a separate genus that clusters together with Bacteroides forsythus and Bacteroides distasonis, two generically misclassified Bacteroides species. The degree of protein similarity correlated with our and published DNA-DNA binding values. Percentage 16S rRNA similarity values of greater than 97% did not guarantee conspecificity. All Capnocytophaga strains had very similar fatty acid contents characterized by significant amounts of 14:0, 15:0 iso (greater than 55%), 16:0, 16:0 30H, and 17:0 iso 30H. PCR-mediated DNA fingerprinting allowed discrimination of most species, although some strains could not be classified efficiently because of DNA polymorphisms.




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