HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by WAUTERS, G. Articles by GRIMONT, P. A. D. Search for Related Content PubMed Articles by WAUTERS, G. Articles by GRIMONT, P. A. D. Agricola Articles by WAUTERS, G. Articles by GRIMONT, P. A. D.

Propionic Acid-Producing Strains Previously Designated as Corynebacterium xerosis, C. minutissimum, C. striatum, and CDC Group I₂ and Group F₂ Coryneforms Belong to the Species Corynebacterium amycolatum

¹Microbiology Unit, University of Louvain, Brussels, Belgium
²Unité des Entérobactéries, INSERM U389, Institut Pasteur, Paris, France

^* Corresponding author. Mailing address: Microbiology Unit, U.C.L./ 5490, avenue Hippocrate, 54, B-1200 Brussels, Belgium. Phone: 32 2 7645490. Fax: 32 2 7649440.

ABSTRACT

Propionic acid-producing Corynebacterium strains that lacked mycolic acids and were formerly identified as Corynebacterium minutissimum, Corynebacterium xerosis, Corynebacterium striatum, and CDC group I² and F² strains were studied to determine their relatedness to Corynebacterium amycolatum. A total of 60 strains were used for phenotypic characterization studies, and 26 of these strains were used for genetic studies. DNA-DNA hybridization experiments performed at 65°C revealed that the levels of relatedness between the propionic acid-producing strains and the type strain of C. amycolatum were more than 70% and that the T_m values ranged from 0 to 5°C (T_m is the difference between the denaturation temperature of a homoduplex and the denaturation temperature of a heteroduplex); these values are consistent with inclusion of these strains in the species C. amycolatum. Currently used conventional tests, such as urease, nitrate reduction, and sugar fermentation tests, were not suitable for accurate identification of C. amycolatum. Phenotypic differentiation of this species from related taxa should be based on the following characteristics in addition to propionic acid production: Lipid requirement, Tween esterase activity, tyrosine clearing, alkaline phosphatase activity, -glucosidase activity, and β-glucuronidase activity.

This article has been cited by other articles:

				G. Wauters, V. Avesani, J. Charlier, M. Janssens, M. Vaneechoutte, and M. Delmee Distribution of Nocardia Species in Clinical Samples and Their Routine Rapid Identification in the Laboratory J. Clin. Microbiol., June 1, 2005; 43(6): 2624 - 2628. [Abstract] [Full Text] [PDF]

				G. Wauters, G. Haase, V. Avesani, J. Charlier, M. Janssens, J. Van Broeck, and M. Delmee Identification of a Novel Brevibacterium Species Isolated from Humans and Description of Brevibacterium sanguinis sp. nov. J. Clin. Microbiol., June 1, 2004; 42(6): 2829 - 2832. [Abstract] [Full Text] [PDF]

				M. Vaneechoutte, P. Kampfer, T. De Baere, E. Falsen, and G. Verschraegen Wautersia gen. nov., a novel genus accommodating the phylogenetic lineage including Ralstonia eutropha and related species, and proposal of Ralstonia [Pseudomonas] syzygii (Roberts et al. 1990) comb. nov. Int J Syst Evol Microbiol, March 1, 2004; 54(2): 317 - 327. [Abstract] [Full Text] [PDF]

				G. Wauters, V. Avesani, K. Laffineur, J. Charlier, M. Janssens, B. Van Bosterhaut, and M. Delmee Brevibacterium lutescens sp. nov., from human and environmental samples Int J Syst Evol Microbiol, September 1, 2003; 53(5): 1321 - 1325. [Abstract] [Full Text] [PDF]

				G. Yague, M. Segovia, and P. L. Valero-Guillen Phospholipid composition of several clinically relevant Corynebacterium species as determined by mass spectrometry: an unusual fatty acyl moiety is present in inositol-containing phospholipids of Corynebacterium urealyticum Microbiology, July 1, 2003; 149(7): 1675 - 1685. [Abstract] [Full Text] [PDF]

				K. Laffineur, V. Avesani, G. Cornu, J. Charlier, M. Janssens, G. Wauters, and M. Delmee Bacteremia Due to a Novel Microbacterium Species in a Patient with Leukemia and Description of Microbacteriumparaoxydans sp. nov. J. Clin. Microbiol., May 1, 2003; 41(5): 2242 - 2246. [Abstract] [Full Text] [PDF]

				G. Wauters, J. Charlier, M. Janssens, and M. Delmée Identification of Arthrobacter oxydans, Arthrobacter luteolus sp. nov., and Arthrobacter albus sp. nov., Isolated from Human Clinical Specimens J. Clin. Microbiol., June 1, 2000; 38(6): 2412 - 2415. [Abstract] [Full Text]

				J. Hommez, L. A. Devriese, M. Vaneechoutte, P. Riegel, P. Butaye, and F. Haesebrouck Identification of Nonlipophilic Corynebacteria Isolated from Dairy Cows with Mastitis J. Clin. Microbiol., April 1, 1999; 37(4): 954 - 957. [Abstract] [Full Text]

				F. N. R. Renaud, M. Dutaur, S. Daoud, D. Aubel, P. Riegel, D. Monget, and J. Freney Differentiation of Corynebacterium amycolatum, C. minutissimum, and C. striatum by Carbon Substrate Assimilation Tests J. Clin. Microbiol., December 1, 1998; 36(12): 3698 - 3702. [Abstract] [Full Text]

				G. Wauters, B. Van Bosterhaut, M. Janssens, and J. Verhaegen Identification of Corynebacterium amycolatum and Other Nonlipophilic Fermentative Corynebacteria of Human Origin J. Clin. Microbiol., May 1, 1998; 36(5): 1430 - 1432. [Abstract] [Full Text]