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Int J Syst Bacteriol 46 (1996), 502-505; DOI 10.1099/00207713-46-2-502
© 1996 Society for General Microbiology
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Identification of Saccharomonospora Strains by the Use of Genomic DNA Fragments and rRNA Gene Probes

JUNG-HOON YOON1,2, HONGIK KIM1, SAM-BONG KIM3, HONG-JOONG KIM1,4, WON YONG KIM*, SUNG TAIK LEE2, MICHAEL GOODFELLOW3 and YONG-HA PARK1,*

1 Bioinformatics & Systematics Laboratory, Korean Collection for Type Cultures, Korea Research Institute of Bioscience and Biotechnology, Korea Institute of Science and Technology, Daeduk Science Park, Taejeon, Republic of Korea
2 Department of Biological Science, Korea Advanced Institute of Science and Technology, Taejeon, Republic of Korea
4 Daesung Microbiology Institute Co., Seoul, Republic of Korea
3 Department of Microbiology, The Medical School, University of Newcastle upon Tyne, Newcastle upon Tyne, NE2 4HH, United Kingdom

* Corresponding author. Phone: 82-42-860-4620. Fax: 82-42-860-4625. Electronic mail address: yhpark{at}gerigw.geri.re.kr.

ABSTRACT

Restriction digestion fragments of DNAs extracted from 14 representative strains of Saccharomonospora azurea, "Saccharomonospora caesia," Saccharomonospora cyanea, Saccharomonospora glauca, and Saccharomonospora viridis and six "Saccharomonospora"-like isolates were separated by electrophoresis, Southern blotted onto nylon membranes, and hybridized by using two rRNA gene probes cloned from Streptomyces griseus subsp. griseus KCTC 9080. The following four restriction endonucleases were used: Bam HI, Sal I, Pvu II, and Xho I. The resultant five ribotype patterns were considered species specific. The genomic diversity revealed by ribotyping indicates that this method can be used to both characterize and identify saccharomonosporae. All of the test strains contained DNA with three rRNA gene clusters.




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