| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Institute of Environmental Science and Engineering, Technical University of Denmark, DK-2800 Lyngby, Denmark
* Corresponding author. Mailing address: Institute of Environmental Science and Engineering, Building 115, Technical University of Denmark, DK-2800 Lyngby, Denmark. Fax: 45 45 93 28 50. Electronic mail address: BKA{at}IMT.DTU.DK.
ABSTRACT
Strain DCB-2T (T = type strain) (T. Madsen and D. Licht, Appl. Environ. Microbiol. 58:2874–2878, 1992) is an anaerobic, spore-forming bacterium that is capable of reductive dechlorination of chlorophenols. The cells of this strain are rod shaped and 3.3 to 6 µm long by 0.6 to 0.7 µm wide and occur singly and in pairs. Short chains are formed. Spores are terminal. This bacterium is motile, and each cell has one or two terminal flagella. Cells in the exponential and stationary phases are gram negative. This organism does not hydrolyze gelatin and is indole positive and catalase negative, and the guanine-plus-cytosine content of its cellular DNA is 47 mol%. The optimum temperature for growth is 37°C. Only pyruvate and tryptophan are used as substrates. Pyruvate and 2,4,6-trichlorophenol are converted to acetate, CO2, and 4-chlorophenol by strain DCB-2T. When grown on pyruvate, this bacterium produces sulfide if thiosulfate or sulfite is added as an electron acceptor. Fe(III) is reduced to Fe(II), but Mn(IV) is not reduced. Sulfate is not reduced to sulfide in the presence of pyruvate or other carbon sources typically used by sulfate-reducing bacteria. Cytochrome c is present, but desulfoviridin is not. DCB-2T reductively dechlorinates 3-chloro-4-hydroxyphenylacetate to 4-hydroxyphenylacetate and conserves energy from the reaction. 16S rRNA sequencing revealed that strain DCB-2T clusters with the Clostridium-Bacillus subphylum and groups with Desulfitobacterium dehalogenans and Desulfotomaculum orientis. Desulfotomaculum orientis does not dechlorinate 2,4,6-trichlorophenol. On the basis of the phylogenetic and physiological differences and similarities of strain DCB-2T, Desulfitobacterium dehalogenans, and Desulfotomaculum orientis, we concluded that DCB-2T belongs to the genus Desulfitobacterium. We propose that strain DCB-2 is the type strain of Desulfitobacterium hafniense sp. nov.
This article has been cited by other articles:
|
|
 |
|
  A. Grostern and E. A. Edwards A 1,1,1-Trichloroethane-Degrading Anaerobic Mixed Microbial Culture Enhances Biotransformation of Mixtures of Chlorinated Ethenes and Ethanes Appl. Envir. Microbiol., December 1, 2006; 72(12): 7849 - 7856. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Gabor, C. S. Verissimo, B. C. Cyran, P. ter Horst, N. P. Meijer, H. Smidt, W. M. de Vos, and J. van der Oost Characterization of CprK1, a CRP/FNR-Type Transcriptional Regulator of Halorespiration from Desulfitobacterium hafniense. J. Bacteriol., April 1, 2006; 188(7): 2604 - 2613. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Lanthier, P. Juteau, F. Lepine, R. Beaudet, and R. Villemur Desulfitobacterium hafniense Is Present in a High Proportion within the Biofilms of a High-Performance Pentachlorophenol-Degrading, Methanogenic Fixed-Film Reactor Appl. Envir. Microbiol., February 1, 2005; 71(2): 1058 - 1065. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. E. Milliken, G. P. Meier, J. E. M. Watts, K. R. Sowers, and H. D. May Microbial Anaerobic Demethylation and Dechlorination of Chlorinated Hydroquinone Metabolites Synthesized by Basidiomycete Fungi Appl. Envir. Microbiol., January 1, 2004; 70(1): 385 - 392. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. W. Saltikov and D. K. Newman Genetic identification of a respiratory arsenate reductase PNAS, September 16, 2003; 100(19): 10983 - 10988. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. P. Goorissen, H. T. S. Boschker, A. J. M. Stams, and T. A. Hansen Isolation of thermophilic Desulfotomaculum strains with methanol and sulfite from solfataric mud pools, and characterization of Desulfotomaculum solfataricum sp. nov. Int J Syst Evol Microbiol, September 1, 2003; 53(5): 1223 - 1229. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Lanthier, B. Tartakovsky, R. Villemur, G. DeLuca, and S. R. Guiot Microstructure of Anaerobic Granules Bioaugmented with Desulfitobacterium frappieri PCP-1 Appl. Envir. Microbiol., August 1, 2002; 68(8): 4035 - 4043. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Niggemyer, S. Spring, E. Stackebrandt, and R. F. Rosenzweig Isolation and Characterization of a Novel As(V)-Reducing Bacterium: Implications for Arsenic Mobilization and the Genus Desulfitobacterium Appl. Envir. Microbiol., December 1, 2001; 67(12): 5568 - 5580. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. A. van de Pas, S. Jansen, C. Dijkema, G. Schraa, W. M. de Vos, and A. J. M. Stams Energy Yield of Respiration on Chloroaromatic Compounds in Desulfitobacterium dehalogenans Appl. Envir. Microbiol., September 1, 2001; 67(9): 3958 - 3963. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. K. Magnuson, M. F. Romine, D. R. Burris, and M. T. Kingsley Trichloroethene Reductive Dehalogenase from Dehalococcoides ethenogenes: Sequence of tceA and Substrate Range Characterization Appl. Envir. Microbiol., December 1, 2000; 66(12): 5141 - 5147. [Abstract] [Full Text]
|
|
|
|
|
|
B. Sun, J. R. Cole, R. A. Sanford, and J. M. Tiedje Isolation and Characterization of Desulfovibrio dechloracetivorans sp. nov., a Marine Dechlorinating Bacterium Growing by Coupling the Oxidation of Acetate to the Reductive Dechlorination of 2-Chlorophenol Appl. Envir. Microbiol., June 1, 2000; 66(6): 2408 - 2413. [Abstract] [Full Text]
|
|
|
|
|
|
J. Gerritse, O. Drzyzga, G. Kloetstra, M. Keijmel, L. P. Wiersum, R. Hutson, M. D. Collins, and J. C. Gottschal Influence of Different Electron Donors and Acceptors on Dehalorespiration of Tetrachloroethene by Desulfitobacterium frappieri TCE1 Appl. Envir. Microbiol., December 1, 1999; 65(12): 5212 - 5221. [Abstract] [Full Text]
|
|
|
|
|
|
T. J. Lie, W. Godchaux, and E. R. Leadbetter Sulfonates as Terminal Electron Acceptors for Growth of Sulfite-Reducing Bacteria (Desulfitobacterium spp.) and Sulfate-Reducing Bacteria: Effects of Inhibitors of Sulfidogenesis Appl. Envir. Microbiol., October 1, 1999; 65(10): 4611 - 4617. [Abstract] [Full Text]
|
|
|
|
|
|
A. W. Boyle, C. D. Phelps, and L. Y. Young Isolation from Estuarine Sediments of a Desulfovibrio Strain Which Can Grow on Lactate Coupled to the Reductive Dehalogenation of 2,4,6-Tribromophenol Appl. Envir. Microbiol., March 1, 1999; 65(3): 1133 - 1140. [Abstract] [Full Text]
|
|
|
|
|
|
T. M. Louie and W. W. Mohn Evidence for a Chemiosmotic Model of Dehalorespiration in Desulfomonile tiedjei DCB-1 J. Bacteriol., January 1, 1999; 181(1): 40 - 46. [Abstract] [Full Text]
|
|
|
|
|
|
D. Dennie, I. Gladu, F. Lépine, R. Villemur, J.-G. Bisaillon, and R. Beaudet Spectrum of the Reductive Dehalogenation Activity of Desulfitobacterium frappieri PCP-1 Appl. Envir. Microbiol., November 1, 1998; 64(11): 4603 - 4606. [Abstract] [Full Text]
|
|
|
|
|
|
C. Hörber, N. Christiansen, E. Arvin, and B. K. Ahring Improved Dechlorinating Performance of Upflow Anaerobic Sludge Blanket Reactors by Incorporation of Dehalospirillum multivorans into Granular Sludge Appl. Envir. Microbiol., May 1, 1998; 64(5): 1860 - 1863. [Abstract] [Full Text]
|
|
|
|
|
|
L. Adrian, W. Manz, U. Szewzyk, and H. Görisch Physiological Characterization of a Bacterial Consortium Reductively Dechlorinating 1,2,3- and 1,2,4-Trichlorobenzene Appl. Envir. Microbiol., February 1, 1998; 64(2): 496 - 503. [Abstract] [Full Text]
|
|
|
|
|
|
M. M. a. J. Wiegel Comparison of Energy and Growth Yields for Desulfitobacterium dehalogenans during Utilization of Chlorophenol and Various Traditional Electron Acceptors Appl. Envir. Microbiol., January 1, 1998; 64(1): 352 - 355. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. Krasotkina, T. Walters, K. A. Maruya, and S. W. Ragsdale Characterization of the B12- and Iron-Sulfur-containing Reductive Dehalogenase from Desulfitobacterium chlororespirans J. Biol. Chem., October 26, 2001; 276(44): 40991 - 40997. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
