| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Woods Hole Oceanographic Institution, Woods Hole, Massachusetts 02543
2 U.S. Biochemical Corporation, Cleveland, Ohio 44128
3 University of South Dakota, Vermillion, South Dakota 57069
* Corresponding author.
ABSTRACT
A new aerobic, obligately chemolithoautotrophic, thermophilic, sulfur-oxidizing bacterium, Thermothrix azorensis, was isolated from a hot spring on Sao Miguel Island in the Azores. The cells of this organism are gram negative, nonsporulating, and rod shaped. Filament formation appears to occur as a response to nonoptimal growth condition. Growth occurs at 63 to 86°C, and the optimum temperature is 76 to 78°C. The optimum pH range for growth is 7.0 to 7.5. The G+C content of the DNA of our isolate is 39.7 mol%. This isolate uses thiosulfate, tetrathionate, hydrogen sulfide, and elemental sulfur as energy sources. Of particular interest are the absence of Calvin cycle enzymes and the initial appearance of sulfide during the lag phase of growth of aerobic cultures grown on elemental sulfur. The subsequent formation of thiosulfate is followed by oxidation of the thiosulfate to sulfate. T. azorensis differs from the only other Thermothrix species that has been described, Thermothrix thiopara, by having higher optimum and maximum growth temperatures, by being an obligate chemolithoautotroph, and by its close but separate position on a 16S rRNA sequence-based phylogenetic tree. Our T. azorensis isolate has been deposited in the American Type Culture Collection as strain ATCC 51754T (T = type strain).
Contribution 8939 of the Woods Hole Oceanographic Institution.
Permanent address: Institute of Microbiology, Russian Academy of Sciences, Prospekt 60-letya Oktyabrya, 7/2, Moscow, 117811 Russia.
This article has been cited by other articles:
|
|
 |
|
  W. Ghosh, A. Bagchi, S. Mandal, B. Dam, and P. Roy Tetrathiobacter kashmirensis gen. nov., sp. nov., a novel mesophilic, neutrophilic, tetrathionate-oxidizing, facultatively chemolithotrophic betaproteobacterium isolated from soil from a temperate orchard in Jammu and Kashmir, India Int J Syst Evol Microbiol, September 1, 2005; 55(5): 1779 - 1787. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Hirayama, K. Takai, F. Inagaki, K. H. Nealson, and K. Horikoshi Thiobacter subterraneus gen. nov., sp. nov., an obligately chemolithoautotrophic, thermophilic, sulfur-oxidizing bacterium from a subsurface hot aquifer Int J Syst Evol Microbiol, January 1, 2005; 55(1): 467 - 472. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Manaia, B. Nogales, and O. C. Nunes Tepidiphilus margaritifer gen. nov., sp. nov., isolated from a thermophilic aerobic digester Int J Syst Evol Microbiol, September 1, 2003; 53(5): 1405 - 1410. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Skirnisdottir, G. O. Hreggvidsson, S. Hjörleifsdottir, V. T. Marteinsson, S. K. Petursdottir, O. Holst, and J. K. Kristjansson Influence of Sulfide and Temperature on Species Composition and Community Structure of Hot Spring Microbial Mats Appl. Envir. Microbiol., July 1, 2000; 66(7): 2835 - 2841. [Abstract] [Full Text]
|
|
|
|
|
|
H. Yamamoto, A. Hiraishi, K. Kato, H. X. Chiura, Y. Maki, and A. Shimizu Phylogenetic Evidence for the Existence of Novel Thermophilic Bacteria in Hot Spring Sulfur-Turf Microbial Mats in Japan Appl. Envir. Microbiol., May 1, 1998; 64(5): 1680 - 1687. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
