HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by SHOONER, F. Articles by TYAGI, R. D. Search for Related Content PubMed PubMed Citation Articles by SHOONER, F. Articles by TYAGI, R. D. Agricola Articles by SHOONER, F. Articles by TYAGI, R. D.

Isolation, Phenotypic Characterization, and Phylogenetic Position of a Novel, Facultatively Autotrophic, Moderately Thermophilic Bacterium, Thiobacillus thermosulfatus sp. nov.

¹ Institut National de la Recherche Scientifique (INRS-Eau), Université du Québec, Sainte-Foy, Québec G1V 4C7, Canada
² Centre de Recherche en Biologie Forestière, Université Laval, Sainte-Foy, Québec G1K 7P4, Canada

^* Corresponding author. Mailing address: Institut National de la Recherche Scientifique (INRS-Eau), Université du Québec, 2700 rue Einstein, C.P. 7500, Sainte-Foy, Québec G1V 4C7, Canada.

ABSTRACT

Thiobacillus thermosulfatus ATCC 51520^T (T = type strain) was isolated from sewage sludge samples enriched with elemental sulfur. The cells of this organism were gram negative, rod shaped, motile, facultatively autotrophic, and strictly aerobic and contained polyphosphate inclusions and polyhedral bodies. During growth on thiosulfate, the following intermediates were produced: tetrathionate, trithionate, and sulfate, and the pH was lowered from neutrality to around 2.5. Autotrophic growth was observed at pH values between 4.3 and 7.8 and at temperatures of 34 to 65°C; optimum growth occurred at pH 5.2 to 5.6 and 50 to 52.5°C. Ubiquinone Q8 was present in the respiratory chain. The DNA contained 61 ± 1 mol% G+C. No denitrification was observed under autotrophic and heterotrophic conditions. The cells produced a glycocalyx during growth in the presence of S⁰. As determined by a 16S rRNA gene sequence analysis, T. thermosulfatus is a distinct species that belongs to the beta subdivision of the Proteobacteria and is closely related phylogenetically to Thiobacillus perometabolis. The GenBank accession number for the complete 16S rRNA gene sequence of T. thermosulfatus is U27839.

This article has been cited by other articles:

				D. P. Kelly, Y. Uchino, H. Huber, R. Amils, and A. P. Wood Reassessment of the phylogenetic relationships of Thiomonas cuprina Int J Syst Evol Microbiol, November 1, 2007; 57(11): 2720 - 2724. [Abstract] [Full Text] [PDF]

				Y. Katayama, Y. Uchino, A. P. Wood, and D. P. Kelly Confirmation of Thiomonas delicata (formerly Thiobacillus delicatus) as a distinct species of the genus Thiomonas Moreira and Amils 1997 with comments on some species currently assigned to the genus. Int J Syst Evol Microbiol, November 1, 2006; 56(Pt 11): 2553 - 2557. [Abstract] [Full Text] [PDF]

				H. Hirayama, K. Takai, F. Inagaki, K. H. Nealson, and K. Horikoshi Thiobacter subterraneus gen. nov., sp. nov., an obligately chemolithoautotrophic, thermophilic, sulfur-oxidizing bacterium from a subsurface hot aquifer Int J Syst Evol Microbiol, January 1, 2005; 55(1): 467 - 472. [Abstract] [Full Text] [PDF]

				C. M. Manaia, B. Nogales, and O. C. Nunes Tepidiphilus margaritifer gen. nov., sp. nov., isolated from a thermophilic aerobic digester Int J Syst Evol Microbiol, September 1, 2003; 53(5): 1405 - 1410. [Abstract] [Full Text] [PDF]